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RESEARCH SPOTLIGHT
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Priming agents transiently reduce the clearance of cell-free DNA to improve liquid biopsies
![Vaccine delivers a boost to T cell therapy](https://images.squarespace-cdn.com/content/v1/6025672c7199794e20d5c7f4/63dc005b-2ad7-44ea-b5cd-677869f062f9/MIT-BoostingTCells-01_0.jpg)
Vaccine delivers a boost to T cell therapy
![A noninvasive test to detect cancer cells and pinpoint their location](https://images.squarespace-cdn.com/content/v1/6025672c7199794e20d5c7f4/cf391ca7-8d35-4b63-914e-c809a4b442f9/MIT-Cancer-Diagnostic-01-press_0.jpg)
A noninvasive test to detect cancer cells and pinpoint their location
![Multiscale profiling of protease activity in cancer](https://images.squarespace-cdn.com/content/v1/6025672c7199794e20d5c7f4/84ede9c6-8c8b-4144-a4d6-9cb7a960f3a8/41467_2022_32988_Fig1.jpg)
Multiscale profiling of protease activity in cancer
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The Irvine Lab has developed a new vaccination strategy to protect mucosal surfaces such as the lungs
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New optical imaging system could be deployed to find tiny tumors
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New vaccine strategy boosts T-cell therapy
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Nanoparticles can turn off genes in bone marrow cells
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Massively parallel pooled screening reveals genomic determinants of nanoparticle delivery
![New technology can detect tiny ovarian tumors](https://images.squarespace-cdn.com/content/v1/6025672c7199794e20d5c7f4/5b26b377-d5e3-4c84-84b1-da5101e7c96a/MIT-Ovarian-Cancer_0.jpg)
New technology can detect tiny ovarian tumors
SELECT PUBLICATIONS
2024
Witten, J., Raji, I., Manan, R.S. et al. Artificial intelligence-guided design of lipid nanoparticles for pulmonary gene therapy. Nat Biotechnol (2024).
Yousefpour, P., Zhang, Y.J., Maiorino, L., et al. Modulation of antigen delivery and lymph node activation in nonhuman primates by saponin adjuvant saponin/monophosphoryl lipid A nanoparticle, PNAS 12 (2024).
Pires, I.S., Hostetler, A., Covarrubias, G., Carlo, I.S., Suggs, J.R., Kim, B.J., Pickering, A.J., Gordon, E., Irvine, D.J. & Hammond, P.T. Charge-Stabilized Nanodiscs as a New Class of Lipid Nanoparticles. Adv. Mat. 29 (2024).
Gordon, A., Li, B., Witten, J., Nguyen, H., Anderson, D.G. Inhalable Dry Powders for Lung mRNA Delivery. Adv. H. Mat. 29 (2024).
Pires, I.S., Suggs, J.R., Carlo, I.S., Sun, D., Hammond, P.T. & Irvine, D.J. Surfactant-Mediated Assembly of Precision-Size Liposomes. Chem. of Mat. 15 (2024).
Kocabiyik, O., Amlashi, P., Vo, A.L., Suh, H., Rodriguez-Aponte, S.A., Dalvie, N.C., Love, J.C., Andrabi, R. & Irvine, D.J. Vaccine targeting to mucosal lymphoid tissues promotes humoral immunity in the gastrointestinal tract. Sci. Adv. 10 (2024).
Hao, L., Boehnke, N., Elledge, S.K., Harzallah, N-S., Zhao, R.T., Cai, E., Feng, Y.X., Neaher, S., Fleming, H.E., Gupta, P.B., Hammond, P.T. & Bhatia, S.N. Targeting and monitoring ovarian cancer invasion with an RNAi and peptide delivery system. PNAS 121 (2024).
Bhatia, S.N. & Dahlman, J.E. RNA Delivery Systems. PNAS 121 (2024).
Van Heest, A.E., Deng, F., Zhao, R.T., Harzallah, N.S., Fleming, H.E., Bhatia, S.N. & Hao, L. CRISPR-Cas-mediated Multianalyte Synthetic Urine Biomarker Test for Portable Diagnostics. J Vis Exp. 8 (2023).
Martin-Alonso , C.,* Tabrizi, S.,* Xiong, Kan, Blewett, T., Sridhar, S., Crnjac, A., Patel, S., An, S., Bekdemir, A., Shea, D., Wang, S-T, Rodriguez-Aponte, S., Naranjo, C.A., Rhoades, J., Kirkpatrick, J.D., Fleming, H.E., Amini, A.P., Golub, T.R., Love, J.C., Bhatia, S.N., Adalsteinsson, V.A. Priming agents transiently reduce the clearance of cell-free DNA to improve liquid biopsies. Science 383 (2024).
Zhong, Q.,* Tan, E.K.W.,* Martin-Alonso, C., Parisi, T., Hao, L., Kirkpatrick, J.D., Fadel, T.R., Fleming, H.E., Jacks, T., & Bhatia, S.N. Inhalable point-of-care urinary diagnostic platform. Sci. Adv. 10 (2024).
*These authors contributed equally to this work.
2023
Kataria, S., Qi, J., Lin, C-W., Li, Z., Dane, E.L. Mahadevan, A., Sacane, I.J., Irvine, D.J. & Belcher, A.M. Noninvasive In Vivo Imaging of T-Cells during Cancer Immunotherapy Using Rare-Earth Nanoparticles. ACS Nano 17,17908–17919 (2023).
Li, B., Jiang, A.Y., Raji, I. et al. Enhancing the immunogenicity of lipid-nanoparticle mRNA vaccines by adjuvanting the ionizable lipid and the mRNA. Nat. Biomed. Eng (2023).
Ma, L., Hostetler, A., Morgan, D.M., Maiorino, L., Sulkaj, I., Whittaker, C.A., Never, A., Piers, I.S., Yousefpour, P., Gregory, J., Qureshi, K., Dye, J., Abraham, W., Sun, H., Li, N., Love, J.C., and Irvine, D.J. Vaccine-boosted CAR T crosstalk with host immunity to reject tumors with antigen heterogeneity. Cell 186, 3148–3165 (2023).
Guimarães, P.G.,* Figueroa-Espada, C.G.,* Riley,R.S., Gong, N., Xue, L., Sewastianikd, T., Dennis, P.S., Loebelf, C., Chungg, A., Shepherd, S.J., Haley, R.M., Hamilton, A.G., El-Mayta, R., Wang, K., Langer, R., Andersong, D.G., Carrascod, R.D., and Mitchell, M.J. In vivo bone marrow microenvironment siRNA delivery using lipid–polymer nanoparticles for multiple myeloma therapy. PNAS 120, e2215711120 (2023).
Zhang, A.Q., Hostetler, A., Chen, L.E. et al. Universal redirection of CAR T cells against solid tumours via membrane-inserted ligands for the CAR. Nat. Biomed. Eng (2023).
Pires, I.S., Ni, K., Melo, M.B., Li, N., Ben-Akiva, E., Maiorino, L., Dye, J., Rodrigues, K.A., Yun, D., Kim, B., Hosn, R.R., Hammond, P.T., Irvine, D.J. Controlled lipid self-assembly for scalable manufacturing of next-generation immune stimulating complexes. Chemical Engineering Journal 464, 142664 (2023).
Rhym, L.H., Manan, R.S., Koller, A. Stephanie, G. & Anderson, D.G. Peptide-encoding mRNA barcodes for the high-throughput in vivo screening of libraries of lipid nanoparticles for mRNA delivery. Nat. Biomed. Eng 7, 901–910 (2023)
Raimondo, T.M., Reed, K., Shi, D., Langer, R. & Anderson, D.G. Delivering the next generation of cancer immunotherapies with RNA. Cell 186, 1535–1540 (2023).
Hao, L., Zhao, R.T., Welch, N.L. et al. CRISPR-Cas-amplified urinary biomarkers for multiplexed and portable cancer diagnostics. Nat. Nanotechnol. 18, 798–807 (2023).
He, Y., Hong, C., Huang, S. et al. STING Protein-Based In Situ Vaccine Synergizes CD4+ T, CD8+ T, and NK Cells for Tumor Eradication. Adv. Health. Mat. 12, 2300688 (2023).
Bhagchandani, S.H., Vohidov, F., Milling, L.E., Tong, E.Y., Brown, C.M., Ramseier, M., Liu, B., Fessenden, T.B., Nguyen, H.V-T., Kiel, G.R., Won, L., Langer, R.S., Spranger, S., Shalek, A.K., Irvine, D.J., Johnson, J.A. Engineering kinetics of TLR7/8 agonist release from bottlebrush prodrugs enables tumor-focused immune stimulation. Science Advances 9 (2023).
Backlund, C.M., Jalili-Firoozinezhad, S., Kim, B. & Irvine, D.J. Biomaterials-Mediated Engineering of the Immune System. Annual Review of Immunology 41, 153–179 (2023).
Hong, C., He, Y., Bowen, P.A., Belcher, A.M., Olsen, B.D. & Hammond, P.T. Engineering a Two-Component Hemostat for the Treatment of Internal Bleeding through Wound-Targeted Crosslinking. Adv. Healthcare Mater. 12 (2023).
Kim, B. Hosn, R.R., Remba, T., Yun, D., Li, N., Abraham, W., Melo, M.B., Cortes, M., Li, B., Zhang, Y., Dong, Y., & Irvine, D.J. Optimization of storage conditions for lipid nanoparticle-formulated self-replicating RNA vaccines. Journal of Control Release 353, 241–253 (2023).
Aung, A., Cui, A. Maiorino, L., Amini, A.P., Gregory, J.R., Bukenya, M., Zhang, Y., Lee, H., Cottrell, C.A., Morgan, D.M., Silva, M., Suh, H., Kirkpatrick, J.D., Amlashi, P., Remba, T., Froehle, L.M., Xiao, S., Abraham, W., Adams, J., Love, J.C., Huyett, P., Kwon, D.S., Hacohen, N., Schief, W.R., Bhatia, S.N., & Irvine, D.J. Low protease activity in B cell follicles promotes retention of intact antigens after immunization. Science 379 (2023).
2022
Barberio, A.E.,* Smith, S.G.,* Pires, I.S., Iyer, S., Reinhardt, F., Melo, M.B., Suh, H., Weinberg, R.A., Irvine, D.J., & Hammond, P.T. Layer-by-layer interleukin-12 nanoparticles drive a safe and effective response in ovarian tumors. Bioengineering & Translational Medicine 379 (2022).
Irvine, D.J., Maus, M.V., Mooney, D.J., & Wong, W.W. The future of engineered immune cell therapies. Science 378 (2022).
Amini, A.P., Kirkpatrick, J.D., Wang, C.S., Jaeger, A.M., Su, S., Naranjo, S., Zhong, Q., Cabana, C.M., Jacks, T., & Bhatia, S.N. Multiscale profiling of protease activity in cancer. Nature Communication 13 (2022).
Backlund, C.M., Holden, R.L., Moynihan, K.D., Garafola, D., Farquhar, C., Mehta, N.K., Maiorino, L., Pham, S., Iorgulescu, J.B., Reardon, D.A., Wu, C.J., Pentelute, B.L., & Irvine, D.J. Cell-penetrating peptides enhance peptide vaccine accumulation and persistence in lymph nodes to drive immunogenicity. PNAS 119 (2022).
Bhatia, S.N., Chen, X., Dobrovolskaia, M.A. & Lammers, T. Cancer nanomedicine. Nature Review Cancer 2022.
Boehnke, N.,* Straehla, J.P.,* Safford, H.C., Kocak, M., Rees, M.G., Ronan, M., Rosenberg, D., Adelmann, C.H., Chivukula, R.R., Nabar, N., Berger, A.G., Lamson, N.G., Cheah, J.H., Li, H., Roth, J.A., Koehler, A.N., & Hammond, P.T. Massively parallel pooled screening reveals genomic determinants of nanoparticle delivery. Science 377 (2022).
Hartwell, B.L., Melo, M.B., Xiao, P., Lemnios, A.A., Li, N., Chang, J.Y.H., Yu, J., Gebre, M.S., Chang, A., Maiorino, L., Carter, C., Moyer, T.J., Dalvie, N.C., Rodriguez-Aponte, S.A., Rodrigues, K.A., Silva, M., Suh, H., Adams, J., Fontenot, J. Love, J.C., Barouch, D.H., Villinger, F., Ruprecht, R.M., & Irvine, D.J. Intranasal vaccination with lipid-conjugated immunogens promotes antigen transmucosal uptake to drive mucosal and systemic immunity. Science Translational Medicine 14 (2022).
Lin, C-W., Huang, S., Colangelo, M., Chen, C., Wong N.C.F., He, Y., Berggren, K. & Belcher, A.M. Surface Plasmon Enhanced Upconversion Fluorescence in Short-Wave Infrared for In Vivo Imaging of Ovarian Cancer. ACS Nano 16, 12930–12940 (2022).
Wamhoff, E.C., Romanov, A., Huang, H., Read, B.J., Ginsburg, E., Knappe, G.A., Kim, H.M., Farrell, N.P., Irvine, D.J., & Bathe, M. Controlling Nuclease Degradation of Wireframe DNA Origami with Minor Groove Binders. ACS Nano 16, 8954-8966 (2022).
Tsedev, U., Lin, C-W., Hess, G.T., Sarkaria, J.N., Lam, F.C., & Belcher, A.M. Phage Particles of Controlled Length and Genome for In Vivo Targeted Glioblastoma Imaging and Therapeutic Delivery. ACS Nano 16, 11676–11691(2022).
Kirkpatrick, J.D., Soleimany, A.P., Dudani, J.S., Liu, H-J., Lam, H.C., Priolo, C., Henske, E.P., & Bhatia, S.N. Protease activity sensors enable real-time treatment response monitoring in lymphangioleiomyomatosis. European Respiratory Journal 59 (2022).
Crosby D., Bhatia, S.N., Brindle, K.M., Coussens, L.M., Dive, C., Emberton, M., Esener, S., Fitzgerald, R.C., Gambhir, S.S., Kuhn, P., Rebbeck, T.R., & Balasubramanian, S. Early Detection of Cancer. Science 375 (2022).
Bekdemir, A., Tanner, E.E.L., Kirkpatrick, J.D., Soleimany, A.P., Mitragotri, S., & Bhatia, S.N. Ionic Liquid-Mediated Transdermal Delivery of Thrombosis-Detecting Nanosensors. Advanced Healthcare Materials 11 (2022).
Dane E.L., Belessiotis-Richards A., Backlund C., Wang J., Hikada K., Milling L.E., Bhagchandani S., Melo M.B., Wu S., Li N., Donahue N., Ni K., Ma L., Okaniwa M., Stevens M.M., Alexander-Katz A., & Irvine D.J., STING agonist delivery by tumour-penetrating lipid nanodiscs primes robust anticancer immunity. Nature Materials 21, 710–720 (2022).
Agarwal Y., Milling, L.E., Chang, J.Y.H., Santollani, L., Sheen, A., Lutz, E.A., Tabet, A., Stinson, J., Ni, K., Rodrigues, K.A., Moyer, T.J., Melo, M.B., Irvine, D.J., & Wittrup, K.D. Intratumourally injected alum-tethered cytokines elicit potent and safer local and systemic anticancer immunity. Nature Biomedical Engineering 6, 129–143 (2022).
Milling, L.E., Garafola, D., Agarwal, Y., Wu, S., Thomas, A., Donahue, N., Adams, J., Thai, N., Suh, H., & Irvine, D.J. Neoadjuvant STING Activation, Extended Half-life IL2, and Checkpoint Blockade Promote Metastasis Clearance via Sustained NK-cell Activation. Cancer Immunology Research 10, 26–39 (2022).
*These authors contributed equally to this work.